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A separate large trial was performed using library domains which were not expected to match with a non-self library fold topology. The results are shown in Figure 4.3 alongside the positive predictions. The fraction of erroneous null predictions decreases with increasing stringency of Z-score threshold. The number of mistaken null predictions with top ranking alignments with is 14 out of a total of 112 (). Thus an estimate for the probability of predicting a fold with this degree of certainty, when none should be predicted, is about 13%.