Pscan DAS TopPred 2 Servers Department Molscript Stockholm University Protein Prediction Servers

Theoretical Chemistry
Protein Prediction Servers

TopPred 2

Topology prediction of membrane proteins

With topology we mean the orientation and location of transmembrane helixes.

Running TopPred 2

Unfortunately the toppres server has been closed. There is a new version available at


The only currently available documentation is the original publication. We appologize for this, and hope to be able to provide a more complete documentation online in future versions of this service.

If you use this service, please cite the following reference:

"Membrane Protein Structure Prediction, Hydrophobicity Analysis and the Positive-inside Rule", Gunnar von Heijne, J. Mol. Biol. (1992) 225, 487-494

A comparison with other methods can be found in:

"Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method", Miklos Cserzo, Erik Wallin, Istvan Simon, Gunnar von Heijne, and Arne Elofsson, to appear in Protein Engineering, vol. 10, no. 6, (1997)