Publications from the von Heijne lab

2013 1 Ojemalm, K., Botelho, S.C., Studle, C. and von Heijne, G. (Epub 2013) Quantitative Analysis of SecYEG-Mediated Insertion of Transmembrane alpha-Helices into the Bacterial Inner Membrane. J Mol Biol (Epub ahead of print)   Link to article PubMed 2 Botelho, S.C., Tatsuta, T., von Heijne, G. and Kim, H. (2013) Dislocation by the m-AAA Protease Increases the Threshold Hydrophobicity for Retention of Transmembrane Helices in the Inner Membrane of Yeast Mitochondria. J Biol Chem 288 (7) : 4792-4798.   Link to article PubMed 2012 3 Lee, H., Min, J., von Heijne, G. and Kim, H. (2012) Glycosylatable GFP as a compartment-specific membrane topology reporter. Biochem Biophys Res Commun 427 (4) : 780-784.   Link to article PubMed 4 Ismail, N., Hedman, R., Schiller, N. and von Heijne, G. (2012) A biphasic pulling force acts on transmembrane helices during translocon-mediated membrane integration. Nat Struct Mol Biol 19 (10) : 1018-1022.   Link to article PubMed 5 Toddo, S., Soderstrom, B., Palombo, I., von Heijne, G., Norholm, M.H. and Daley, D.O. (2012) Application of split-green fluorescent protein for topology mapping membrane proteins in Escherichia coli. Protein Sci 21 (10) : 1571-1576.   Link to article PubMed 6 Lloris-Garcera, P., Bianchi, F., Slusky, J.S., Seppala, S., Daley, D.O. and von Heijne, G. (2012) Antiparallel Dimers of the Small Multidrug Resistance Protein EmrE Are More Stable Than Parallel Dimers. J Biol Chem 287 (31) : 26052-26059.   Link to article PubMed 7 Galian, C., Bjorkholm, P., Bulleid, N. and von Heijne, G. (2012) Efficient glycosylphosphatidylinositol (GPI) modification of membrane proteins requires a C-terminal anchoring signal of marginal hydrophobicity. J Biol Chem 287 (20) : 16399-16409.   Link to article PubMed 8 Ojemalm, K., Halling, K.K., Nilsson, I. and von Heijne, G. (2012) Orientational Preferences of Neighboring Helices Can Drive ER Insertion of a Marginally Hydrophobic Transmembrane Helix. Mol Cell 45 (4) : 529-540.   Link to article PubMed 9 Contreras, F.X., Ernst, A.M., Haberkant, P., Bjorkholm, P., Lindahl, E., Gonen, B., Tischer, C., Elofsson, A., von Heijne, G., Thiele, C., Pepperkok, R., Wieland, F. and Brugger, B. (2012) Molecular recognition of a single sphingolipid species by a protein's transmembrane domain. Nature 481 (7382) : 525-529.   Link to article PubMed 10 Skoog, K., Soderstrom, B., Widengren, J., von Heijne, G. and Daley, D.O. (2012) Sequential Closure of the Cytoplasm and Then the Periplasm during Cell Division in Escherichia coli. J Bacteriol 194 (3) : 584-586.   Link to article PubMed 11 Norholm, M.H., Light, S., Virkki, M.T., Elofsson, A., von Heijne, G. and Daley, D.O. (2012) Manipulating the genetic code for membrane protein production: What have we learnt so far? Biochim Biophys Acta 1818 (4) : 1091-1096.   Link to article PubMed 2011 12 Petersen, T.N., Brunak, S., von Heijne, G. and Nielsen, H. (2011) SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods 8 (10) : 785-786.   Link to article PubMed 13 von Heijne, G. (2011) Introduction to theme "membrane protein folding and insertion". Annu Rev Biochem 80: 157-160.   Link to article PubMed 14 Norholm, M.H., Shulga, Y.V., Aoki, S., Epand, R.M. and von Heijne, G. (2011) Flanking residues help determine whether a hydrophobic segment adopts a monotopic or bitopic topology in the endoplasmic reticulum membrane. J Biol Chem 286 (28) : 25284-25290.   Link to article PubMed 15 Ojemalm, K., Higuchi, T., Jiang, Y., Langel, U., Nilsson, I., White, S.H., Suga, H. and von Heijne, G. (2011) Apolar surface area determines the efficiency of translocon-mediated membrane-protein integration into the endoplasmic reticulum. Proc Natl Acad Sci U S A 108 (31) : E359-64.   Link to article PubMed 16 von Heijne, G. (2011) Membrane proteins: from bench to bits. Biochem Soc Trans 39 (3) : 747-750.   Link to article PubMed 17 Osterberg, M., Calado Botelho, S., von Heijne, G. and Kim, H. (2011) Charged flanking residues control the efficiency of membrane insertion of the first transmembrane segment in yeast mitochondrial Mgm1p. FEBS Lett 585 (8) : 1238-1242.   Link to article PubMed 18 Botelho, S.C., Osterberg, M., Reichert, A.S., Yamano, K., Bjorkholm, P., Endo, T., von Heijne, G. and Kim, H. (2011) TIM23-mediated insertion of transmembrane alpha-helices into the mitochondrial inner membrane. EMBO J 30 (6) : 1003-1011.   Link to article PubMed 19 Norholm, M.H., Cunningham, F., Deber, C.M. and von Heijne, G. (2011) Converting a marginally hydrophobic soluble protein into a membrane protein. J Mol Biol 407 (1) : 171-179.   Link to article PubMed 2010 20 Seppala, S., Slusky, J.S., Lloris-Garcera, P., Rapp, M. and von Heijne, G. (2010) Control of membrane protein topology by a single C-terminal residue. Science 328 (5986) : 1698-1700.   Link to article PubMed 21 Fagerberg, L., Jonasson, K., von Heijne, G., Uhlen, M. and Berglund, L. (2010) Prediction of the human membrane proteome. Proteomics 10 (6) : 1141-1149.   Link to article PubMed 22 Stromqvist, J., Skoog, K., Daley, D.O., Widengren, J. and von Heijne, G. (2010) Estimating Z-ring radius and contraction in dividing Escherichia coli. Mol Microbiol 76 (1) : 151-158.   Link to article PubMed 23 Kauko, A., Hedin, L.E., Thebaud, E., Cristobal, S., Elofsson, A. and von Heijne, G. (2010) Repositioning of Transmembrane alpha-Helices during Membrane Protein Folding. J Mol Biol 397 (1) : 190-201.   Link to article PubMed 24 Sakata, S., Kurokawa, T., Norholm, M.H., Takagi, M., Okochi, Y., von Heijne, G. and Okamura, Y. (2010) Functionality of the voltage-gated proton channel truncated in S4. Proc Natl Acad Sci U S A 107 (5) : 2313-2318.   Link to article PubMed 25 Daniels, R., Mellroth, P., Bernsel, A., Neiers, F., Normark, S., von Heijne, G. and Henriques-Normark, B. (2010) Disulfide Bond Formation and Cysteine Exclusion in Gram-positive Bacteria. J Biol Chem 285 (5) : 3300-3309.   Link to article PubMed 26 Hedin, L.E., Ojemalm, K., Bernsel, A., Hennerdal, A., Illergard, K., Enquist, K., Kauko, A., Cristobal, S., von Heijne, G., Lerch-Bader, M., Nilsson, I. and Elofsson, A. (2010) Membrane Insertion of Marginally Hydrophobic Transmembrane Helices Depends on Sequence Context. J Mol Biol 396 (1) : 221-229.   Link to article PubMed 2009 27 Jaud, S., Fernandez-Vidal, M., Nilsson, I., Meindl-Beinker, N.M., Hubner, N.C., Tobias, D.J., von Heijne, G. and White, S.H. (2009) Insertion of short transmembrane helices by the Sec61 translocon. Proc Natl Acad Sci U S A 106 (28) : 11588-11593.   Link to article PubMed 28 Hessa, T., Reithinger, J.H., von Heijne, G. and Kim, H. (2009) Analysis of transmembrane helix integration in the endoplasmic reticulum in S. cerevisiae. J Mol Biol 386 (5) : 1222-1228.     PubMed 29 Enquist, K., Fransson, M., Boekel, C., Bengtsson, I., Geiger, K., Lang, L., Pettersson, A., Johansson, S., von Heijne, G. and Nilsson, I. (2009) Membrane-integration characteristics of two ABC transporters, CFTR and P-glycoprotein. J Mol Biol 387 (5) : 1153-1164.   Link to article PubMed 30 Juncker, A.S., Jensen, L.J., Pierleoni, A., Bernsel, A., Tress, M.L., Bork, P., von Heijne, G., Valencia, A., Ouzounis, C.A., Casadio, R. and Brunak, S. (2009) Sequence-based feature prediction and annotation of proteins. Genome Biol 10 (2) : 206.   Link to article PubMed 31 Hessa, T., Reithinger, J.H., von Heijne, G. and Kim, H. (2009) Quantitative analysis of transmembrane helix integration in the endoplasmic reticulum in S. cerevisiae J Mol Biol 386 (5) : 1222-1228.   Link to article   2008 32 Lundin, C., Kim, H., Nilsson, I., White, S.H. and von Heijne, G. (2008) Molecular code for protein insertion in the endoplasmic reticulum membrane is similar for N(in)-C(out) and N(out)-C(in) transmembrane helices. Proc Natl Acad Sci U S A 105 (41) : 15702-15707.   Link to article PubMed 33 von Heijne, G. and Rees, D. (2008) Membranes: reading between the lines. Curr Opin Struct Biol 18 (4) : 403-405.   Link to article PubMed 34 Cassel, M., Seppala, S. and von Heijne, G. (2008) Confronting fusion protein-based membrane protein topology mapping with reality: the Escherichia coli ClcA H+/Cl- exchange transporter. J Mol Biol 381 (4) : 860-866.   Link to article PubMed 35 White, S.H. and von Heijne, G. (2008) How translocons select transmembrane helices. Annu Rev Biophys 37: 23-42.   Link to article PubMed 36 Bernsel, A., Viklund, H., Falk, J., Lindahl, E., von Heijne, G. and Elofsson, A. (2008) Prediction of membrane-protein topology from first principles. Proc Natl Acad Sci U S A 105 (20) : 7177-7181.   Link to article PubMed 37 Drew, D., Newstead, S., Sonoda, Y., Kim, H., von Heijne, G. and Iwata, S. (2008) GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. Nat Protoc 3 (5) : 784-798.   Link to article PubMed 38 Lerch-Bader, M., Lundin, C., Kim, H., Nilsson, I. and von Heijne, G. (2008) Contribution of positively charged flanking residues to the insertion of transmembrane helices into the endoplasmic reticulum. Proc Natl Acad Sci U S A 105 (11) : 4127-4132.   Link to article PubMed 2007 39 Hessa, T., Meindl-Beinker, N.M., Bernsel, A., Kim, H., Sato, Y., Lerch-Bader, M., Nilsson, I., White, S.H. and von Heijne, G. (2007) Molecular code for transmembrane-helix recognition by the Sec61 translocon. Nature 450 (7172) : 1026-1030.   Link to article PubMed 40 Xie, K., Hessa, T., Seppala, S., Rapp, M., Heijne, G. and Dalbey, R.E. (2007) Features of transmembrane segments that promote the lateral release from the translocase into the lipid phase. Biochemistry 46 (51) : 15153-15161.   Link to article PubMed 41 Lundin, C., Kall, L., Kreher, S.A., Kapp, K., Sonnhammer, E.L., Carlson, J.R., Heijne, G. and Nilsson, I. (2007) Membrane topology of the Drosophila OR83b odorant receptor. FEBS Lett 581 (29) : 5601-5604.   Link to article PubMed 42 Granseth, E., Seppala, S., Rapp, M., Daley, D.O. and Von Heijne, G. (2007) Membrane protein structural biology - How far can the bugs take us? (Review). Mol Membr Biol 24 (5) : 329-332.   Link to article PubMed 43 Newstead, S., Kim, H., von Heijne, G., Iwata, S. and Drew, D. (2007) High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 104 (35) : 13936-13941.   Link to article PubMed 44 Lundin, C., Johansson, S., Johnson, A.E., Naslund, J., von Heijne, G. and Nilsson, I. (2007) Stable insertion of Alzheimer Abeta peptide into the ER membrane strongly correlates with its length. FEBS Lett 581 (20) : 3809-3813.   Link to article PubMed 45 Stenberg, F., von Heijne, G. and Daley, D.O. (2007) Assembly of the Cytochrome bo(3) Complex. J Mol Biol 371 (3) : 765-773.   Link to article PubMed 46 Elofsson, A. and von Heijne, G. (2007) Membrane protein structure: prediction versus reality. Annu Rev Biochem 76: 125-140.   Link to article PubMed 47 von Heijne, G. (2007) Membrane proteins up for grabs. Nat Biotechnol 25 (6) : 646-647.   Link to article PubMed 48 von Heijne, G. (2007) The membrane protein universe: what's out there and why bother? J Intern Med 261 (6) : 543-557.   Link to article PubMed 49 Zhang, L., Sato, Y., Hessa, T., von Heijne, G., Lee, J.K., Kodama, I., Sakaguchi, M. and Uozumi, N. (2007) Contribution of hydrophobic and electrostatic interactions to the membrane integration of the Shaker K+ channel voltage sensor domain. Proc Natl Acad Sci U S A 104 (20) : 8263-8268.   Link to article PubMed 50 Emanuelsson, O., Brunak, S., von Heijne, G. and Nielsen, H. (2007) Locating proteins in the cell using TargetP, SignalP and related tools. Nat Protoc 2 (4) : 953-971.   Link to article PubMed 51 von Heijne, G. (2007) Formation of Transmembrane Helices In Vivo--Is Hydrophobicity All that Matters? J Gen Physiol 129 (5) : 353-356.   Link to article PubMed 52 Rapp, M., Seppala, S., Granseth, E. and von Heijne, G. (2007) Emulating membrane protein evolution by rational design. Science 315 (5816) : 1282-1284.   Link to article PubMed 2006 53 von Heijne, G. (2006) Membrane-protein topology. Nat Rev Mol Cell Biol 7 (12) : 909-918.   Link to article PubMed 54 Meindl-Beinker, N.M., Lundin, C., Nilsson, I., White, S.H. and von Heijne, G. (2006) Asn- and Asp-mediated interactions between transmembrane helices during translocon-mediated membrane protein assembly. EMBO Rep 7 (11) : 1111-1116.   Link to article PubMed 55 Kim, H., Melen, K., Osterberg, M. and von Heijne, G. (2006) A global topology map of the Saccharomyces cerevisiae membrane proteome. Proc Natl Acad Sci U S A 103 (30) : 11142-11147.   Link to article PubMed 56 Osterberg, M., Kim, H., Warringer, J., Melen, K., Blomberg, A. and von Heijne, G. (2006) Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 103 (30) : 11148-11153.   Link to article PubMed 57 Amico, M., Finelli, M., Rossi, I., Zauli, A., Elofsson, A., Viklund, H., von Heijne, G., Jones, D., Krogh, A., Fariselli, P., Luigi Martelli, P. and Casadio, R. (2006) PONGO: a web server for multiple predictions of all-alpha transmembrane proteins. Nucleic Acids Res 34 (Web Server issue) : W169-72.   Link to article PubMed 58 Mackinnon, R. and von Heijne, G. (2006) Membranes. Curr Opin Struct Biol 16 (4) : 431.   Link to article PubMed 59 Lundin, C., Nordstrom, R., Wagner, K., Windpassinger, C., Andersson, H., von Heijne, G. and Nilsson, I. (2006) Membrane topology of the human seipin protein. FEBS Lett 580 (9) : 2281-2284.   Link to article PubMed 60 Marani, P., Wagner, S., Baars, L., Genevaux, P., de Gier, J.W., Nilsson, I., Casadio, R. and von Heijne, G. (2006) New Escherichia coli outer membrane proteins identified through prediction and experimental verification. Protein Sci 15 (4) : 884-889.   Link to article PubMed 61 Rapp, M., Granseth, E., Seppala, S. and von Heijne, G. (2006) Identification and evolution of dual-topology membrane proteins. Nat Struct Mol Biol 13 (2) : 112-116.   Link to article PubMed 2005 62 Villarejo, A., Buren, S., Larsson, S., Dejardin, A., Monne, M., Rudhe, C., Karlsson, J., Jansson, S., Lerouge, P., Rolland, N., von Heijne, G., Grebe, M., Bako, L. and Samuelsson, G. (2005) Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast. Nat Cell Biol 7 (12) : 1224-1231.   Link to article PubMed 63 White, S.H. and von Heijne, G. (2005) Do protein-lipid interactions determine the recognition of transmembrane helices at the ER translocon? Biochem Soc Trans 33 (Pt 5) : 1012-1015.   Link to article PubMed 64 Freites, J.A., Tobias, D.J., von Heijne, G. and White, S.H. (2005) Interface connections of a transmembrane voltage sensor. Proc Natl Acad Sci U S A 102 (42) : 15059-15064.   Link to article PubMed 65 Karamyshev, A.L., Kelleher, D.J., Gilmore, R., Johnson, A.E., von Heijne, G. and Nilsson, I. (2005) Mapping the interaction of the STT3 subunit of the oligosaccharyl transferase complex with nascent polypeptide chains. J Biol Chem 280 (49) : 40489-40493.   Link to article PubMed 66 von Heijne, G. (2005) Helices on the move. Nat Struct Mol Biol 12 (10) : 834-835.   Link to article PubMed 67 Pasche, B., Knobloch, T.J., Bian, Y., Liu, J., Phukan, S., Rosman, D., Kaklamani, V., Baddi, L., Siddiqui, F.S., Frankel, W., Prior, T.W., Schuller, D.E., Agrawal, A., Lang, J., Dolan, M.E., Vokes, E.E., Lane, W.S., Huang, C.C., Caldes, T., Di Cristofano, A., Hampel, H., Nilsson, I., von Heijne, G., Fodde, R., Murty, V.V., de la Chapelle, A. and Weghorst, C.M. (2005) Somatic acquisition and signaling of TGFBR1*6A in cancer. JAMA 294 (13) : 1634-1646.   Link to article PubMed 68 Medigue, C., Krin, E., Pascal, G., Barbe, V., Bernsel, A., Bertin, P.N., Cheung, F., Cruveiller, S., D'Amico, S., Duilio, A., Fang, G., Feller, G., Ho, C., Mangenot, S., Marino, G., Nilsson, J., Parrilli, E., Rocha, E.P., Rouy, Z., Sekowska, A., Tutino, M.L., Vallenet, D., von Heijne, G. and Danchin, A. (2005) Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125. Genome Res 15 (10) : 1325-1335.   Link to article PubMed 69 Luirink, J., von Heijne, G., Houben, E. and de Gier, J.W. (2005) Biogenesis of inner membrane proteins in Escherichia coli. Annu Rev Microbiol 59: 329-355.   Link to article PubMed 70 Granseth, E., Daley, D.O., Rapp, M., Melen, K. and von Heijne, G. (2005) Experimentally constrained topology models for 51,208 bacterial inner membrane proteins. J Mol Biol 352 (3) : 489-494.   Link to article PubMed 71 Stenberg, F., Chovanec, P., Maslen, S.L., Robinson, C.V., Ilag, L.L., von Heijne, G. and Daley, D.O. (2005) Protein complexes of the Escherichia coli cell envelope. J Biol Chem 280 (41) : 34409-34419.   Link to article PubMed 72 von Heijne, G. (2005) Microbiology. Translocation of anthrax toxin: lord of the rings. Science 309 (5735) : 709-710.   Link to article PubMed 73 Laudon, H., Hansson, E.M., Melen, K., Bergman, A., Farmery, M.R., Winblad, B., Lendahl, U., von Heijne, G. and Naslund, J. (2005) A nine-transmembrane domain topology for presenilin 1. J Biol Chem 280 (42) : 35352-35360.   Link to article PubMed 74 White, S.H. and von Heijne, G. (2005) Transmembrane helices before, during, and after insertion. Curr Opin Struct Biol 15 (4) : 378-386.   Link to article PubMed 75 Nilsson, J., Persson, B. and von Heijne, G. (2005) Comparative analysis of amino acid distributions in integral membrane proteins from 107 genomes. Proteins 60 (4) : 606-616.   Link to article PubMed 76 Bernsel, A. and Von Heijne, G. (2005) Improved membrane protein topology prediction by domain assignments. Protein Sci 14 (7) : 1723-1728.   Link to article PubMed 77 Daley, D.O., Rapp, M., Granseth, E., Melen, K., Drew, D. and von Heijne, G. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 (5726) : 1321-1323.   Link to article PubMed 78 Kim, H., von Heijne, G. and Nilsson, I. (2005) Membrane topology of the STT3 subunit of the oligosaccharyl transferase complex. J Biol Chem 280 (21) : 20261-20267.   Link to article PubMed 79 Tie, J.K., Nicchitta, C., von Heijne, G. and Stafford, D.W. (2005) Membrane topology mapping of vitamin K epoxide reductase by in vitro translation/cotranslocation. J Biol Chem 280 (16) : 16410-16416.   Link to article PubMed 80 Hessa, T., White, S.H. and von Heijne, G. (2005) Membrane insertion of a potassium-channel voltage sensor. Science 307 (5714) : 1427.   Link to article PubMed 81 Ding, B., Kull, B., Liu, Z., Mottagui-Tabar, S., Thonberg, H., Gu, H.F., Brookes, A.J., Grundemar, L., Karlsson, C., Hamsten, A., Arner, P., Ostenson, C.G., Efendic, S., Monne, M., von Heijne, G., Eriksson, P. and Wahlestedt, C. (2005) Human neuropeptide Y signal peptide gain-of-function polymorphism is associated with increased body mass index: possible mode of function. Regul Pept 127 (1-3) : 45-53.   Link to article PubMed 82 Hessa, T., Kim, H., Bihlmaier, K., Lundin, C., Boekel, J., Andersson, H., Nilsson, I., White, S.H. and von Heijne, G. (2005) Recognition of transmembrane helices by the endoplasmic reticulum translocon. Nature 433 (7024) : 377-381.   Link to article PubMed 83 Granseth, E., von Heijne, G. and Elofsson, A. (2005) A study of the membrane-water interface region of membrane proteins. J Mol Biol 346 (1) : 377-385.   Link to article PubMed 84 Monne, M., Hessa, T., Thissen, L. and von Heijne, G. (2005) Competition between neighboring topogenic signals during membrane protein insertion into the ER. FEBS J 272 (1) : 28-36.   Link to article PubMed 2004 85 Kerje, S., Sharma, P., Gunnarsson, U., Kim, H., Bagchi, S., Fredriksson, R., Schutz, K., Jensen, P., von Heijne, G., Okimoto, R. and Andersson, L. (2004) The Dominant white, Dun and Smoky color variants in chicken are associated with insertion/deletion polymorphisms in the PMEL17 gene. Genetics 168 (3) : 1507-1518.   Link to article PubMed 86 Bendtsen, J.D., Nielsen, H., von Heijne, G. and Brunak, S. (2004) Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340 (4) : 783-795.     PubMed 2003 87 Hermansson, M. and von Heijne, G. (2003) Inter-helical hydrogen bond formation during membrane protein integration into the ER membrane. J Mol Biol 334 (4) : 803-809.     PubMed 88 Westerlund, I., Von Heijne, G. and Emanuelsson, O. (2003) LumenP--a neural network predictor for protein localization in the thylakoid lumen. Protein Sci 12 (10) : 2360-2366.   Link to article PubMed 89 Juncker, A.S., Willenbrock, H., Von Heijne, G., Brunak, S., Nielsen, H. and Krogh, A. (2003) Prediction of lipoprotein signal peptides in Gram-negative bacteria. Protein Sci 12 (8) : 1652-1662.   Link to article PubMed 90 Emanuelsson, O., Elofsson, A., von Heijne, G. and Cristobal, S. (2003) In silico prediction of the peroxisomal proteome in fungi, plants and animals. J Mol Biol 330 (2) : 443-456.     PubMed 91 Nilsson, I., Johnson, A.E. and von Heijne, G. (2003) How hydrophobic is alanine? J Biol Chem 278 (32) : 29389-29393.     PubMed 92 Von Heijne, G. (2003) Membrane protein assembly in vivo. Adv Protein Chem 63: 1-18.     PubMed 93 Hessa, T., Monne, M. and von Heijne, G. (2003) Stop-transfer efficiency of marginally hydrophobic segments depends on the length of the carboxy-terminal tail. EMBO Rep 4 (2) : 178-183.     PubMed 2002 94 Nilsson, J., Persson, B. and Von Heijne, G. (2002) Prediction of partial membrane protein topologies using a consensus approach. Protein Sci 11 (12) : 2974-2980.   Link to article PubMed 95 Blomqvist, G., Alvarsson, M., Grill, V., Von Heijne, G., Ingvar, M., Thorell, J.O., Stone-Elander, S., Widen, L. and Ekberg, K. (2002) Effect of acute hyperketonemia on the cerebral uptake of ketone bodies in nondiabetic subjects and IDDM patients. Am J Physiol Endocrinol Metab 283 (1) : E20-8.   Link to article PubMed 96 Nilsson, I., Johnson, A.E. and von Heijne, G. (2002) Cleavage of a tail-anchored protein by signal peptidase. FEBS Lett 516 (1-3) : 106-108.     PubMed 97 Hedman, M., Deloof, H., Von Heijne, G. and Elofsson, A. (2002) Improved detection of homologous membrane proteins by inclusion of information from topology predictions. Protein Sci 11 (3) : 652-658.     PubMed 2001 98 Hermansson, M., Monne, M. and von Heijne, G. (2001) Formation of helical hairpins during membrane protein integration into the endoplasmic reticulum membrane. Role of the N and C-terminal flanking regions. J Mol Biol 313 (5) : 1171-1179.     PubMed 99 Monne, M. and von Heijne, G. (2001) Effects of 'hydrophobic mismatch' on the location of transmembrane helices in the ER membrane. FEBS Lett 496 (2-3) : 96-100.     PubMed 100 Saaf, A., Baars, L. and von Heijne, G. (2001) The internal repeats in the Na+/Ca2+ exchanger-related Escherichia coli protein YrbG have opposite membrane topologies. J Biol Chem 276 (22) : 18905-18907.     PubMed 2000 101 Mingarro, I., Nilsson, I., Whitley, P. and von Heijne, G. (2000) Different conformations of nascent polypeptides during translocation across the ER membrane. BMC Cell Biol 1: 3.     PubMed 102 Nilsson, J., Persson, B. and von Heijne, G. (2000) Consensus predictions of membrane protein topology. FEBS Lett 486 (3) : 267-269.     PubMed 103 Chin, C.N. and von Heijne, G. (2000) Charge pair interactions in a model transmembrane helix in the ER membrane. J Mol Biol 303 (1) : 1-5.     PubMed 104 Killian, J.A. and von Heijne, G. (2000) How proteins adapt to a membrane-water interface. Trends Biochem Sci 25 (9) : 429-434.     PubMed 105 Saaf, A., Hermansson, M. and von Heijne, G. (2000) Formation of cytoplasmic turns between two closely spaced transmembrane helices during membrane protein integration into the ER membrane. J Mol Biol 301 (1) : 191-197.     PubMed 106 Emanuelsson, O., Nielsen, H., Brunak, S. and von Heijne, G. (2000) Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol 300 (4) : 1005-1016.     PubMed 107 Hasler, U., Greasley, P.J., von Heijne, G. and Geering, K. (2000) Determinants of topogenesis and glycosylation of type II membrane proteins. Analysis of Na,K-ATPase beta 1 AND beta 3 subunits by glycosylation mapping. J Biol Chem 275 (37) : 29011-29022.     PubMed 108 Nilsson, I. and von Heijne, G. (2000) Glycosylation efficiency of Asn-Xaa-Thr sequons depends both on the distance from the C terminus and on the presence of a downstream transmembrane segment. J Biol Chem 275 (23) : 17338-17343.     PubMed 109 Nilsson, I., Witt, S., Kiefer, H., Mingarro, I. and von Heijne, G. (2000) Distant downstream sequence determinants can control N-tail translocation during protein insertion into the endoplasmic reticulum membrane. J Biol Chem 275 (9) : 6207-6213.     PubMed 110 Scotti, P.A., Urbanus, M.L., Brunner, J., de Gier, J.W., von Heijne, G., van der Does, C., Driessen, A.J., Oudega, B. and Luirink, J. (2000) YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase. EMBO J 19 (4) : 542-549.   Link to article PubMed 1999 111 von Heijne, G. (1999) Recent advances in the understanding of membrane protein assembly and structure. Q Rev Biophys 32 (4) : 285-307.     PubMed 112 Armulik, A., Nilsson, I., von Heijne, G. and Johansson, S. (1999) Determination of the border between the transmembrane and cytoplasmic domains of human integrin subunits. J Biol Chem 274 (52) : 37030-37034.     PubMed 113 Devoto, A., Piffanelli, P., Nilsson, I., Wallin, E., Panstruga, R., von Heijne, G. and Schulze-Lefert, P. (1999) Topology, subcellular localization, and sequence diversity of the Mlo family in plants. J Biol Chem 274 (49) : 34993-35004.     PubMed 114 Monne, M., Nilsson, I., Elofsson, A. and von Heijne, G. (1999) Turns in transmembrane helices: determination of the minimal length of a "helical hairpin" and derivation of a fine-grained turn propensity scale. J Mol Biol 293 (4) : 807-814.     PubMed 115 von Heijne, G. (1999) A Day in the Life of Dr K. or How I Learned to Stop Worrying and Love Lysozyme: a tragedy in six acts. J Mol Biol 293 (2) : 367-379.     PubMed 116 Monne, M., Gafvelin, G., Nilsson, R. and von Heijne, G. (1999) N-tail translocation in a eukaryotic polytopic membrane protein: synergy between neighboring transmembrane segments. Eur J Biochem 263 (1) : 264-269.     PubMed 117 Braun, P. and von Heijne, G. (1999) The aromatic residues Trp and Phe have different effects on the positioning of a transmembrane helix in the microsomal membrane. Biochemistry 38 (30) : 9778-9782.     PubMed 118 Fredriksson, A., Johnstrom, P., Thorell, J.O., von Heijne, G., Hassan, M., Eksborg, S., Kogner, P., Borgstrom, P., Ingvar, M. and Stone-Elander, S. (1999) In vivo evaluation of the biodistribution of 11C-labeled PD153035 in rats without and with neuroblastoma implants. Life Sci 65 (2) : 165-174.     PubMed 119 Saaf, A., Johansson, M., Wallin, E. and von Heijne, G. (1999) Divergent evolution of membrane protein topology: the Escherichia coli RnfA and RnfE homologues. Proc Natl Acad Sci U S A 96 (15) : 8540-8544.     PubMed 120 Cristobal, S., Scotti, P., Luirink, J., von Heijne, G. and de Gier, J.W. (1999) The signal recognition particle-targeting pathway does not necessarily deliver proteins to the sec-translocase in Escherichia coli. J Biol Chem 274 (29) : 20068-20070.     PubMed 121 Cristobal, S., de Gier, J.W., Nielsen, H. and von Heijne, G. (1999) Competition between Sec- and TAT-dependent protein translocation in Escherichia coli. EMBO J 18 (11) : 2982-2990.   Link to article PubMed 122 Emanuelsson, O., Nielsen, H. and von Heijne, G. (1999) ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci 8 (5) : 978-984.     PubMed 123 Monne, M., Hermansson, M. and von Heijne, G. (1999) A turn propensity scale for transmembrane helices. J Mol Biol 288 (1) : 141-145.   Link to article PubMed 124 Nielsen, H., Brunak, S. and von Heijne, G. (1999) Machine learning approaches for the prediction of signal peptides and other protein sorting signals. Protein Eng 12 (1) : 3-9.     PubMed 125 van Geest, M., Nilsson, I., von Heijne, G. and Lolkema, J.S. (1999) Insertion of a bacterial secondary transport protein in the endoplasmic reticulum membrane. J Biol Chem 274 (5) : 2816-2823.     PubMed 1998 126 de Gier, J.W., Scotti, P.A., Saaf, A., Valent, Q.A., Kuhn, A., Luirink, J. and von Heijne, G. (1998) Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli. Proc Natl Acad Sci U S A 95 (25) : 14646-14651.     PubMed 127 Nilsson, I. and von Heijne, G. (1998) Breaking the camel's back: proline-induced turns in a model transmembrane helix. J Mol Biol 284 (4) : 1185-1189.   Link to article PubMed 128 Monne, M., Nilsson, I., Johansson, M., Elmhed, N. and von Heijne, G. (1998) Positively and negatively charged residues have different effects on the position in the membrane of a model transmembrane helix. J Mol Biol 284 (4) : 1177-1183.   Link to article PubMed 129 Nilsson, I., Saaf, A., Whitley, P., Gafvelin, G., Waller, C. and von Heijne, G. (1998) Proline-induced disruption of a transmembrane alpha-helix in its natural environment. J Mol Biol 284 (4) : 1165-1175.   Link to article PubMed 130 von Heijne, G. (1998) Life and death of a signal peptide. Nature 396 (6707) : 111, 113.   Link to article PubMed 131 Ota, K., Sakaguchi, M., von Heijne, G., Hamasaki, N. and Mihara, K. (1998) Forced transmembrane orientation of hydrophilic polypeptide segments in multispanning membrane proteins. Mol Cell 2 (4) : 495-503.     PubMed 132 Saaf, A., Monne, M., de Gier, J.W. and von Heijne, G. (1998) Membrane topology of the 60-kDa Oxa1p homologue from Escherichia coli. J Biol Chem 273 (46) : 30415-30418.     PubMed 133 von Heijne, G. (1998) Structural aspects of transmembrane alpha-helices. Acta Physiol Scand Suppl 643: 17-19.     PubMed 134 Sonnhammer, E.L., von Heijne, G. and Krogh, A. (1998) A hidden Markov model for predicting transmembrane helices in protein sequences. Proc Int Conf Intell Syst Mol Biol 6: 175-182.     PubMed 135 Seshadri, K., Garemyr, R., Wallin, E., von Heijne, G. and Elofsson, A. (1998) Architecture of beta-barrel membrane proteins: analysis of trimeric porins. Protein Sci 7 (9) : 2026-2032.     PubMed 136 van Klompenburg, W., Paetzel, M., de Jong, J.M., Dalbey, R.E., Demel, R.A., von Heijne, G. and de Kruijff, B. (1998) Phosphatidylethanolamine mediates insertion of the catalytic domain of leader peptidase in membranes. FEBS Lett 431 (1) : 75-79.     PubMed 137 Ingvar, M., Ghatan, P.H., Wirsen-Meurling, A., Risberg, J., Von Heijne, G., Stone-Elander, S. and Ingvar, D.H. (1998) Alcohol activates the cerebral reward system in man. J Stud Alcohol 59 (3) : 258-269.     PubMed 138 Wallin, E. and von Heijne, G. (1998) Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms. Protein Sci 7 (4) : 1029-1038.     PubMed 139 Valent, Q.A., Scotti, P.A., High, S., de Gier, J.W., von Heijne, G., Lentzen, G., Wintermeyer, W., Oudega, B. and Luirink, J. (1998) The Escherichia coli SRP and SecB targeting pathways converge at the translocon. EMBO J 17 (9) : 2504-2512.   Link to article PubMed 140 Saaf, A., Wallin, E. and von Heijne, G. (1998) Stop-transfer function of pseudo-random amino acid segments during translocation across prokaryotic and eukaryotic membranes. Eur J Biochem 251 (3) : 821-829.     PubMed 141 Schneider, G., Sjoling, S., Wallin, E., Wrede, P., Glaser, E. and von Heijne, G. (1998) Feature-extraction from endopeptidase cleavage sites in mitochondrial targeting peptides. Proteins 30 (1) : 49-60.     PubMed 1997 142 Valent, Q.A., de Gier, J.W., von Heijne, G., Kendall, D.A., ten Hagen-Jongman, C.M., Oudega, B. and Luirink, J. (1997) Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor. Mol Microbiol 25 (1) : 53-64.     PubMed 143 Nielsen, H., Engelbrecht, J., Brunak, S. and von Heijne, G. (1997) A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Int J Neural Syst 8 (5-6) : 581-599.     PubMed 144 Braun, P., Persson, B., Kaback, H.R. and von Heijne, G. (1997) Alanine insertion scanning mutagenesis of lactose permease transmembrane helices. J Biol Chem 272 (47) : 29566-29571.     PubMed 145 Mingarro, I., von Heijne, G. and Whitley, P. (1997) Membrane-protein engineering. Trends Biotechnol 15 (10) : 432-437.   Link to article PubMed 146 Mingarro, I., Elofsson, A. and von Heijne, G. (1997) Helix-helix packing in a membrane-like environment. J Mol Biol 272 (4) : 633-641.   Link to article PubMed 147 van Klompenburg, W., Ridder, A.N., van Raalte, A.L., Killian, A.J., von Heijne, G. and de Kruijff, B. (1997) In vitro membrane integration of leader peptidase depends on the Sec machinery and anionic phospholipids and can occur post-translationally. FEBS Lett 413 (1) : 109-114.     PubMed 148 Cserzo, M., Wallin, E., Simon, I., von Heijne, G. and Elofsson, A. (1997) Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method. Protein Eng 10 (6) : 673-676.     PubMed 149 van Klompenburg, W., Nilsson, I., von Heijne, G. and de Kruijff, B. (1997) Anionic phospholipids are determinants of membrane protein topology. EMBO J 16 (14) : 4261-4266.     PubMed 150 Claros, M.G., Brunak, S. and von Heijne, G. (1997) Prediction of N-terminal protein sorting signals. Curr Opin Struct Biol 7 (3) : 394-398.     PubMed 151 De Gier, J.W., Valent, Q.A., Von Heijne, G. and Luirink, J. (1997) The E. coli SRP: preferences of a targeting factor. FEBS Lett 408 (1) : 1-4.     PubMed 152 Mothes, W., Heinrich, S.U., Graf, R., Nilsson, I., von Heijne, G., Brunner, J. and Rapoport, T.A. (1997) Molecular mechanism of membrane protein integration into the endoplasmic reticulum. Cell 89 (4) : 523-533.     PubMed 153 von Heijne, G. (1997) Getting greasy: how transmembrane polypeptide segments integrate into the lipid bilayer. Mol Microbiol 24 (2) : 249-253.     PubMed 154 Wallin, E., Tsukihara, T., Yoshikawa, S., von Heijne, G. and Elofsson, A. (1997) Architecture of helix bundle membrane proteins: an analysis of cytochrome c oxidase from bovine mitochondria. Protein Sci 6 (4) : 808-815.     PubMed 155 Nielsen, H., Engelbrecht, J., Brunak, S. and von Heijne, G. (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 10 (1) : 1-6.     PubMed 156 Gafvelin, G., Sakaguchi, M., Andersson, H. and von Heijne, G. (1997) Topological rules for membrane protein assembly in eukaryotic cells. J Biol Chem 272 (10) : 6119-6127.     PubMed 1996 157 von Heijne, G. (1996) Principles of membrane protein assembly and structure. Prog Biophys Mol Biol 66 (2) : 113-139.     PubMed 158 de Gier, J.W., Mansournia, P., Valent, Q.A., Phillips, G.J., Luirink, J. and von Heijne, G. (1996) Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle. FEBS Lett 399 (3) : 307-309.     PubMed 159 Glick, B.S. and Von Heijne, G. (1996) Saccharomyces cerevisiae mitochondria lack a bacterial-type sec machinery. Protein Sci 5 (12) : 2651-2652.     PubMed 160 Kiefer, H., Krieger, J., Olszewski, J.D., Von Heijne, G., Prestwich, G.D. and Breer, H. (1996) Expression of an olfactory receptor in Escherichia coli: purification, reconstitution, and ligand binding. Biochemistry 35 (50) : 16077-16084.     PubMed 161 Andersson, H., Nilsson, I. and von Heijne, G. (1996) Calnexin can interact with N-linked glycans located close to the endoplasmic reticulum membrane. FEBS Lett 397 (2-3) : 321-324.     PubMed 162 Johansson, M. and von Heijne, G. (1996) Membrane topology of Kch, a putative K+ channel from Escherichia coli. J Biol Chem 271 (42) : 25912-25915.     PubMed 163 Nielsen, H., Engelbrecht, J., von Heijne, G. and Brunak, S. (1996) Defining a similarity threshold for a functional protein sequence pattern: the signal peptide cleavage site. Proteins 24 (2) : 165-177.   Link to article PubMed 164 Mingarro, I., Whitley, P., Lemmon, M.A. and von Heijne, G. (1996) Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix: a rapid way to map helix-helix interactions in integral membrane proteins. Protein Sci 5 (7) : 1339-1341.     PubMed 165 Whitley, P., Grahn, E., Kutay, U., Rapoport, T.A. and von Heijne, G. (1996) A 12-residue-long polyleucine tail is sufficient to anchor synaptobrevin to the endoplasmic reticulum membrane. J Biol Chem 271 (13) : 7583-7586.     PubMed 166 Whitley, P., Nilsson, I.M. and von Heijne, G. (1996) A nascent secretory protein may traverse the ribosome/endoplasmic reticulum translocase complex as an extended chain. J Biol Chem 271 (11) : 6241-6244.     PubMed 1995 167 Dalbey, R.E., Kuhn, A. and von Heijne, G. (1995) Directionality in protein translocation across membranes: the N-tail phenomenon. Trends Cell Biol 5 (10) : 380-383.     PubMed 168 van Klompenburg, W., Whitley, P., Diemel, R., von Heijne, G. and de Kruijff, B. (1995) A quantitative assay to determine the amount of signal peptidase I in E. coli and the orientation of membrane vesicles. Mol Membr Biol 12 (4) : 349-353.     PubMed 169 Whitley, P., Saaf, A., Gafvelin, G., Johansson, M., Wallin, E. and von Heijne, G. (1995) Membrane protein anchors and polytopic determinants. Biochem Soc Trans 23 (4) : 965-967.     PubMed 170 Wallin, E. and von Heijne, G. (1995) Properties of N-terminal tails in G-protein coupled receptors: a statistical study. Protein Eng 8 (7) : 693-698.     PubMed 171 Whitley, P., Gafvelin, G. and von Heijne, G. (1995) SecA-independent translocation of the periplasmic N-terminal tail of an Escherichia coli inner membrane protein. Position-specific effects on translocation of positively charged residues and construct J Biol Chem 270 (50) : 29831-29835.     PubMed 172 Saaf, A., Andersson, H., Gafvelin, G. and von Heijne, G. (1995) SecA-dependence of the translocation of a large periplasmic loop in the Escherichia coli MalF inner membrane protein is a function of sequence context. Mol Membr Biol 12 (2) : 209-215.     PubMed 173 von Heijne, G. (1995) Membrane protein assembly: rules of the game. Bioessays 17 (1) : 25-30.   Link to article PubMed 174 von Heijne, G. (1995) Protein sorting signals: simple peptides with complex functions. EXS 73: 67-76.     PubMed 1994 175 von Heijne, G. (1994) Sec-independent protein insertion into the inner E. coli membrane. A phenomenon in search of an explanation. FEBS Lett 346 (1) : 69-72.     PubMed 176 Andersson, H. and von Heijne, G. (1994) Membrane protein topology: effects of delta mu H+ on the translocation of charged residues explain the 'positive inside' rule. EMBO J 13 (10) : 2267-2272.     PubMed 177 Gafvelin, G. and von Heijne, G. (1994) Topological "frustration" in multispanning E. coli inner membrane proteins. Cell 77 (3) : 401-412.     PubMed 178 von Heijne, G. (1994) Signals for protein targeting into and across membranes. Subcell Biochem 22: 1-19.     PubMed 179 Nilsson, I., Whitley, P. and von Heijne, G. (1994) The COOH-terminal ends of internal signal and signal-anchor sequences are positioned differently in the ER translocase. J Cell Biol 126 (5) : 1127-1132.     PubMed 180 Andersson, H. and von Heijne, G. (1994) Positively charged residues influence the degree of SecA dependence in protein translocation across the E. coli inner membrane. FEBS Lett 347 (2-3) : 169-172.     PubMed 181 Cao, G., Cheng, S., Whitley, P., von Heijne, G., Kuhn, A. and Dalbey, R.E. (1994) Synergistic insertion of two hydrophobic regions drives Sec-independent membrane protein assembly. J Biol Chem 269 (43) : 26898-26903.     PubMed 182 Whitley, P., Zander, T., Ehrmann, M., Haardt, M., Bremer, E. and von Heijne, G. (1994) Sec-independent translocation of a 100-residue periplasmic N-terminal tail in the E. coli inner membrane protein proW. EMBO J 13 (19) : 4653-4661.     PubMed 183 von Heijne, G. (1994) Membrane proteins: from sequence to structure. Annu Rev Biophys Biomol Struct 23: 167-192.     PubMed 184 Hirsch, S., Muckel, E., Heemeyer, F., von Heijne, G. and Soll, J. (1994) A receptor component of the chloroplast protein translocation machinery. Science 266 (5193) : 1989-1992.     PubMed 185 Whitley, P., Nilsson, I. and von Heijne, G. (1994) De novo design of integral membrane proteins. Nat Struct Biol 1 (12) : 858-862.     PubMed 186 Claros, M.G. and von Heijne, G. (1994) TopPred II: an improved software for membrane protein structure predictions. Comput Appl Biosci 10 (6) : 685-686.     PubMed 1993 187 Johansson, M., Nilsson, I. and von Heijne, G. (1993) Positively charged amino acids placed next to a signal sequence block protein translocation more efficiently in Escherichia coli than in mammalian microsomes. Mol Gen Genet 239 (1-2) : 251-256.     PubMed 188 Nilsson, I.M. and von Heijne, G. (1993) Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane. J Biol Chem 268 (8) : 5798-5801.     PubMed 189 Andersson, H. and von Heijne, G. (1993) Sec dependent and sec independent assembly of E. coli inner membrane proteins: the topological rules depend on chain length. EMBO J 12 (2) : 683-691.     PubMed 190 Nilsson, I.M., Gafvelin, G. and von Heijne, G. (1993) Different sec-requirements for signal peptide cleavage and protein translocation in a model E. coli protein. FEBS Lett 318 (1) : 7-10.     PubMed 191 Wallin, E., Wettergren, C., Hedman, F. and von Heijne, G. (1993) Fast Needleman-Wunsch scanning of sequence databanks on a massively parallel computer. Comput Appl Biosci 9 (1) : 117-118.     PubMed 192 Andersson, H. and von Heijne, G. (1993) Position-specific Asp-Lys pairing can affect signal sequence function and membrane protein topology. J Biol Chem 268 (28) : 21389-21393.     PubMed 193 Whitley, P. and von Heijne, G. (1993) The DsbA-DsbB system affects the formation of disulfide bonds in periplasmic but not in intramembraneous protein domains. FEBS Lett 332 (1-2) : 49-51.     PubMed 194 Whitley, P., Nilsson, L. and von Heijne, G. (1993) Three-dimensional model for the membrane domain of Escherichia coli leader peptidase based on disulfide mapping. Biochemistry 32 (33) : 8534-8539.     PubMed 195 Sipos, L. and von Heijne, G. (1993) Predicting the topology of eukaryotic membrane proteins. Eur J Biochem 213 (3) : 1333-1340.     PubMed 1992 196 von Heijne, G. (1992) Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule. J Mol Biol 225 (2) : 487-494.     PubMed 197 Gavel, Y. and von Heijne, G. (1992) The distribution of charged amino acids in mitochondrial inner-membrane proteins suggests different modes of membrane integration for nuclearly and mitochondrially encoded proteins. Eur J Biochem 205 (3) : 1207-1215.     PubMed 198 Nilsson, I. and von Heijne, G. (1992) A signal peptide with a proline next to the cleavage site inhibits leader peptidase when present in a sec-independent protein. FEBS Lett 299 (3) : 243-246.     PubMed 199 Cramer, W.A., Engelman, D.M., Von Heijne, G. and Rees, D.C. (1992) Forces involved in the assembly and stabilization of membrane proteins. FASEB J 6 (15) : 3397-3402.     PubMed 200 Dalbey, R.E. and Von Heijne, G. (1992) Signal peptidases in prokaryotes and eukaryotes--a new protease family. Trends Biochem Sci 17 (11) : 474-478.     PubMed 201 von Heijne, G. (1992) Cleavage-site motifs in protein targeting sequences. Genet Eng (N Y) 14: 1-11.     PubMed 202 Andersson, H., Bakker, E. and von Heijne, G. (1992) Different positively charged amino acids have similar effects on the topology of a polytopic transmembrane protein in Escherichia coli. J Biol Chem 267 (3) : 1491-1495.     PubMed 1991 203 Gavel, Y., Steppuhn, J., Herrmann, R. and von Heijne, G. (1991) The 'positive-inside rule' applies to thylakoid membrane proteins. FEBS Lett 282 (1) : 41-46.     PubMed 204 Wilson, I.B., Gavel, Y. and von Heijne, G. (1991) Amino acid distributions around O-linked glycosylation sites. Biochem J 275 ( Pt 2): 529-534.     PubMed 205 von Heijne, G. (1991) Proline kinks in transmembrane alpha-helices. J Mol Biol 218 (3) : 499-503.     PubMed 206 Nilsson, I. and von Heijne, G. (1991) A de novo designed signal peptide cleavage cassette functions in vivo. J Biol Chem 266 (6) : 3408-3410.     PubMed 207 von Heijne, G. and Nishikawa, K. (1991) Chloroplast transit peptides. The perfect random coil? FEBS Lett 278 (1) : 1-3.     PubMed 208 Andersson, H. and von Heijne, G. (1991) A 30-residue-long "export initiation domain" adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli. Proc Natl Acad Sci U S A 88 (21) : 9751-9754.     PubMed 209 von Heijne, G., Liljestrom, P., Mikus, P., Andersson, H. and Ny, T. (1991) The efficiency of the uncleaved secretion signal in the plasminogen activator inhibitor type 2 protein can be enhanced by point mutations that increase its hydrophobicity. J Biol Chem 266 (23) : 15240-15243.     PubMed 210 von Heijne, G. (1991) Computer analysis of DNA and protein sequences. Eur J Biochem 199 (2) : 253-256.     PubMed 1990 211 Franzen, L.G., Rochaix, J.D. and von Heijne, G. (1990) Chloroplast transit peptides from the green alga Chlamydomonas reinhardtii share features with both mitochondrial and higher plant chloroplast presequences. FEBS Lett 260 (2) : 165-168.     PubMed 212 Gavel, Y. and von Heijne, G. (1990) Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering. Protein Eng 3 (5) : 433-442.     PubMed 213 Gavel, Y. and von Heijne, G. (1990) A conserved cleavage-site motif in chloroplast transit peptides. FEBS Lett 261 (2) : 455-458.     PubMed 214 Gavel, Y. and von Heijne, G. (1990) Cleavage-site motifs in mitochondrial targeting peptides. Protein Eng 4 (1) : 33-37.     PubMed 215 Von Heijne, G. (1990) [Intracellular protein transport--the cell as a mail carrier] Lakartidningen 87 (50) : 4311-4314.     PubMed 216 von Heijne, G. (1990) Protein targeting signals. Curr Opin Cell Biol 2 (4) : 604-608.     PubMed 217 Bilgin, N., Lee, J.I., Zhu, H.Y., Dalbey, R. and von Heijne, G. (1990) Mapping of catalytically important domains in Escherichia coli leader peptidase. EMBO J 9 (9) : 2717-2722.     PubMed 218 von Heijne, G. (1990) The signal peptide. J Membr Biol 115 (3) : 195-201.     PubMed 219 Nilsson, I. and von Heijne, G. (1990) Fine-tuning the topology of a polytopic membrane protein: role of positively and negatively charged amino acids. Cell 62 (6) : 1135-1141.     PubMed 220 von Heijne, G. and Manoil, C. (1990) Membrane proteins: from sequence to structure. Protein Eng 4 (2) : 109-112.     PubMed 221 von Heijne, G. (1990) Imports, exports, and quality control. Intracellular Routing of Protein Molecules, sponsored by the Karolinska Institute, Center for Biotechnology, Huddinge, Sweden, May 18-19, 1990. New Biol 2 (7) : 609-611.     PubMed 1989 222 von Heijne, G. (1989) Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues. Nature 341 (6241) : 456-458.   Link to article PubMed 223 von Heijne, G. (1989) The structure of signal peptides from bacterial lipoproteins. Protein Eng 2 (7) : 531-534.     PubMed 224 von Heijne, G., Steppuhn, J. and Herrmann, R.G. (1989) Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem 180 (3) : 535-545.     PubMed 225 von Heijne, G. and Abrahmsen, L. (1989) Species-specific variation in signal peptide design. Implications for protein secretion in foreign hosts. FEBS Lett 244 (2) : 439-446.     PubMed 1988 226 Gavel, Y., Nilsson, L. and von Heijne, G. (1988) Mitochondrial targeting sequences. Why 'non-amphiphilic' peptides may still be amphiphilic. FEBS Lett 235 (1-2) : 173-177.     PubMed 227 von Heijne, G. (1988) Getting sense out of sequence data. Nature 333 (6174) : 605-607.   Link to article PubMed 228 von Heijne, G. (1988) Transcending the impenetrable: how proteins come to terms with membranes. Biochim Biophys Acta 947 (2) : 307-333.     PubMed 229 von Heijne, G., Wickner, W. and Dalbey, R.E. (1988) The cytoplasmic domain of Escherichia coli leader peptidase is a "translocation poison" sequence. Proc Natl Acad Sci U S A 85 (10) : 3363-3366.     PubMed 230 von Heijne, G. and Gavel, Y. (1988) Topogenic signals in integral membrane proteins. Eur J Biochem 174 (4) : 671-678.     PubMed 1987 231 von Heijne, G. and Segrest, J.P. (1987) The leader peptides from bacteriorhodopsin and halorhodopsin are potential membrane-spanning amphipathic helices. FEBS Lett 213 (2) : 238-240.     PubMed 232 von Heijne, G. (1987) SIGPEP: a sequence database for secretory signal peptides. Protein Seq Data Anal 1 (1) : 41-42.     PubMed 233 von Heijne, G. and Uhlen, M. (1987) Homology to region X from staphylococcal protein A is not unique to cell surface proteins. J Theor Biol 127 (3) : 373-376.     PubMed 234 von Heijne, G., Blomberg, C. and Liljenstrom, H. (1987) Theoretical modelling of protein synthesis. J Theor Biol 125 (1) : 1-14.     PubMed 235 Liljenstrom, H. and von Heijne, G. (1987) Translation rate modification by preferential codon usage: intragenic position effects. J Theor Biol 124 (1) : 43-55.     PubMed 1986 236 von Heijne, G. (1986) Towards a comparative anatomy of N-terminal topogenic protein sequences. J Mol Biol 189 (1) : 239-242.     PubMed 237 von Heijne, G. (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 14 (11) : 4683-4690.   Link to article PubMed 238 von Heijne, G. (1986) Net N-C charge imbalance may be important for signal sequence function in bacteria. J Mol Biol 192 (2) : 287-290.     PubMed 239 von Heijne, G. (1986) Why mitochondria need a genome. FEBS Lett 198 (1) : 1-4.     PubMed 240 Flinta, C., Persson, B., Jornvall, H. and von Heijne, G. (1986) Sequence determinants of cytosolic N-terminal protein processing. Eur J Biochem 154 (1) : 193-196.     PubMed 241 von Heijne, G. (1986) Mitochondrial targeting sequences may form amphiphilic helices. EMBO J 5 (6) : 1335-1342.     PubMed 1985 242 Persson, B., Flinta, C., von Heijne, G. and Jornvall, H. (1985) Structures of N-terminally acetylated proteins. Eur J Biochem 152 (3) : 523-527.     PubMed 243 von Heijne, G. (1985) Signal sequences. The limits of variation. J Mol Biol 184 (1) : 99-105.     PubMed 244 Liljenstrom, H., von Heijne, G., Blomberg, C. and Johansson, J. (1985) The tRNA cycle and its relation to the rate of protein synthesis. Eur Biophys J 12 (2) : 115-119.     PubMed 245 von Heijne, G. (1985) Ribosome-SRP-signal sequence interactions. The relay helix hypothesis. FEBS Lett 190 (1) : 1-5.     PubMed 246 von Heijne, G. (1985) "Theoretical experiments" on amino acid and nucleotide sequences: three case-studies. Biomed Biochim Acta 44 (6) : 903-911.     PubMed 1984 247 von Heijne, G. (1984) Analysis of the distribution of charged residues in the N-terminal region of signal sequences: implications for protein export in prokaryotic and eukaryotic cells. EMBO J 3 (10) : 2315-2318.     PubMed 248 von Heijne, G. (1984) How signal sequences maintain cleavage specificity. J Mol Biol 173 (2) : 243-251.     PubMed 1983 249 Flinta, C., von Heijne, G. and Johansson, J. (1983) Helical sidedness and the distribution of polar residues in trans-membrane helices. J Mol Biol 168 (1) : 193-196.     PubMed 250 von Heijne, G. (1983) Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem 133 (1) : 17-21.     PubMed 1982 251 von Heijne, G. (1982) Signal sequences are not uniformly hydrophobic. J Mol Biol 159 (3) : 537-541.     PubMed 252 von Heijne, G. and Savageau, M.A. (1982) RNA splicing: advantages of parallel processing. J Theor Biol 98 (4) : 563-574.     PubMed 253 von Heijne, G. (1982) A theoretical study of the attenuation control mechanism. J Theor Biol 97 (2) : 227-238.     PubMed 1981 254 von Heijne, G. (1981) Membrane proteins: the amino acid composition of membrane-penetrating segments. Eur J Biochem 120 (2) : 275-278.     PubMed 255 von Heijne, G. (1981) On the hydrophobic nature of signal sequences. Eur J Biochem 116 (2) : 419-422.     PubMed 1980 256 von Heijne, G. (1980) Trans-membrane translocation of proteins. A detailed physico-chemical analysis. Eur J Biochem 103 (3) : 431-438.     PubMed 257 von Heijne, G. (1980) A sequence correlation between oppositely charged residues in secreted proteins. Biochem Biophys Res Commun 93 (1) : 82-86.     PubMed 1979 258 von Heijne, G. and Blomberg, C. (1979) The concentration dependence of the error frequencies and some related quantities in protein synthesis. J Theor Biol 78 (1) : 113-120.     PubMed 259 von Heijne, G. and Blomberg, C. (1979) Trans-membrane translocation of proteins. The direct transfer model. Eur J Biochem 97 (1) : 175-181.     PubMed 1978 260 von Heijne, G., Leimar, O. and Blomberg, C. (1978) On the emergence of new function in primitive proteins. J Theor Biol 75 (2) : 167-180.     PubMed 261 von Heijne, G. and Blomberg, C. (1978) Early evolution of cellular electron transport: molecular models for the ferredoxin-rubredoxin-flavodoxin region. Orig Life 9 (1) : 27-37.     PubMed 262 von Heijne, G., Nilsson, L. and Blomberg, C. (1978) Models for mRNA translation: theory versus experiment. Eur J Biochem 92 (2) : 397-402.     PubMed 1977 263 von Heijne, G. and Blomberg, C. (1977) The beta structure: inter-strand correlations. J Mol Biol 117 (3) : 821-824.     PubMed 264 von Heijne, G., Nilsson, L. and Blomberg, C. (1977) Translation and messenger RNA secondary structure. J Theor Biol 68 (3) : 321-329.     PubMed 1976 265 von Heijne, G. and Blomberg, C. (1976) Kinetic aspects of the DNA helix - cruciform transition. J Theor Biol 63 (2) : 347-353.     PubMed 1973 266 von Heijne, G. and Teder, A. (1973) Kinetics of the decomposition of aqueous chlorine dioxide solutions. Acta Chem Scand 27 (10) : 4018-4019.     PubMed